Raman scattering is used to obtain information about the structure and properties of molecules from their vibrational transitions. Renishaw inVia system is equipped with the Leica DMIRBE inverted optical microscope. inVia Raman Microscope offers two laser excitation sources at 514 nm and 785 nm, respectively. This highly collimated, monochromatic light illuminates on a sample through a Leica objective and the Raman scattered light from the sample is collected by an objective, analyzed through inVia Spectrometer, and focused on a CCD camera.