Our lab studies the molecular basis of innate immunity, a primordial antimicrobial defense mechanism that is conserved from invertebrates to higher animals. In particular, we focus on a class of intracellular immune sensors called the NOD-like receptors (NLRs) that play essential roles in host defense against pathogens, but also carry genetic variants that are associated with several human autoinflammatory and autoimmune syndromes. We use an ensemble systems immunology approach involving high-throughput experimental, computational, and engineering tools to investigate NLR signaling and regulation at the transcriptional, post-transcriptional, cellular, and organismal scales in various infectious and complex disease contexts. Our eventual goal is to develop strategies for engineering the immune system to treat diseases related to immune dysfunction.