The Posner Lab research focuses on need-driven research projects including point-of-care in-vitro diagnostics and medical device development. We develop and validate nucleic acid, immuno, and enzyme-based diagnostics for a range of infectious disease applications, including HIV and HCV. We specialize in novel point-of-care sample preparation strategies –such as paper microfluidic devices, enzyme chemistry, and isothermal amplification– for low-resource environments that allow complex samples (such as blood, urine, etc.) to be adapted to assays that typically require many human handling steps. We use device fabrication, novel chemistry, electrokinetics, optics, mobile phones, and machine learning to develop integrated and quantitative diagnostics at the point-of-use. Our lab also develops novel enzymatic assays (RESTRICT and INTEGRITY) for measuring pharmaceutical drug level measurement of anti-retorviral compounds for personalized treatment of infectious diseases (HIV), therapeutic medication adherence, long acting injectables, and validation of clinical trials for vaccines.